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Inoculation of Slash Pine Seedlings with Stored Basidiospores of Cronartium fusiforme. Thomas Miller, Associate Plant Pathologist, Southeastern Forest Experiment Station, Forest Service, USDA, Forestry Sciences Laboratory, Athens, Georgia 30601. Phytopathology 60:1773-1774. Accepted for publication 14 July 1970. DOI: 10.1094/Phyto-60-1773.

Precast basidiospores of Cronartium fusiforme were concentrated onto Millipore filters. Inoculation instruments made from glass capillary tubes were used to transfer basidiospores from filters to specific points on slash pine seedlings. Infections occurred on hypocotyls, cotyledons, stems, and secondary needles. Basidiospores stored on filters at 5 C have retained infectivity for 8 weeks.

Additional keywords: Fusiform rust, Pinus elliottii var. elliottii.