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Properties of a Strain of Strawberry Latent Ringspot Virus Isolated from Sweet Cherry Growing in Ontario. Wayne R. Allen, Research Station, Canada Department of Agriculture, Vineland Station, Ontario, Canada; T. R. Davidson(2), and M. R. Briscoe(3). (2)(3)Research Station, Canada Department of Agriculture, Vineland Station, Ontario, Canada. Phytopathology 60:1262-1265. Accepted for publication 22 March 1970. DOI: 10.1094/Phyto-60-1262.

Strawberry latent ringspot virus (SLRV) was isolated from leaves, flower parts, and embryos of a 16-year-old Bing sweet cherry tree growing in the Niagara Peninsula of Ontario, Canada. It has a dilution end point of 104, a thermal inactivation point of 54 C, a particle diam of 29-32 mµ (average 30 mµ), a sedimentation coefficient of 134 S, a buoyant density of 1.274 g/cm3, and a nucleic acid content of 30%. Seed transmission of SLRV in Chenopodium quinoa ranged from 63 to 100%; infection through seed resulted in stunting, deformed leaves, and chlorotic patterns. Peach was infected by approach grafting to Chenopodium; no symptoms resulted other than possible stunting. Absence of the vector, Xiphinema diversicaudatum, from field soil in Ontario and the failure to detect the virus in other plantings of Bing cherry suggest that SLRV was imported.

Additional keywords: Properties, purification, density-gradient centrifugation.