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Spatial and Temporal Distribution of Geminiviruses in Leafhoppers of the Genus Cicadulina Monitored by Conventional and Quantitative Polymerase Chain Reaction

January 2002 , Volume 92 , Number  1
Pages  65 - 74

Jean-Michel Lett , Martine Granier , Isabelle Hippolyte , Martial Grondin , Monique Royer , Stéphane Blanc , Bernard Reynaud , and Michel Peterschmitt

First, second, and eighth authors: CIRAD-AMIS, Laboratoire de Virologie, TA 40/02, 34398 Montpellier Cedex 5, France; third author: CIRAD-AMIS, Laboratoire BIOTROP-ALBHADES, TA 40/03, 34398 Montpellier Cedex 5, France; fourth and seventh authors: CIRAD-AMIS, Pôle de Protection des Plantes, 97410 Saint-Pierre, La Réunion, France; fifth author: CIRAD-AMIS, Laboratoire IGEPAM, TA 40/02, 34398 Montpellier Cedex 5, France; and sixth author: INRA-CNRS, Laboratoire de biologie cellulaire et moléculaire, 30380 Saint-Christol les Alès, France

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Accepted for publication 10 September 2001.

Spatial and temporal distribution of Maize streak virus (MSV, family Geminiviridae, genus Mastrevirus) was monitored in the vector species Cicadulina mbila and the nonvector species C. chinaï using conventional and real-time quantitative polymerase chain reaction. Sustained feeding on MSV-infected plants showed that virus accumulation reaches a maximum in C. chinaï, but not in C. mbila. After a 3-day acquisition access feeding period (AAP), MSV was detected in the gut, the hemolymph, and the head of C. mbila, but only in the gut of C. chinaï. Similarly, Digitaria streak virus (genus Mastrevirus), which is not transmitted by either of the two species, was only detected in the gut. MSV was detected in the hemolymph of C. mbila 3 h after the beginning of the AAP. Although viral DNA progressively decreases in the vector and nonvector species after a 3-day AAP, MSV DNA remained stable in the salivary glands of C. mbila.

Additional keywords: circulative transmission, insect compartments, transmission barrier, viral kinetics.

© 2002 The American Phytopathological Society