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Survey of Prunus necrotic ringspot virus in Rose and Its Variability in Rose and Prunus spp.

January 2001 , Volume 91 , Number  1
Pages  84 - 91

Benoît Moury , Loïc Cardin , Jean-Paul Onesto , Thierry Candresse , and Alain Poupet

First, second, third, and fifth authors: INRA, Unité de recherche Santé végétale et environnement, Villa Thuret, 62 bd du Cap, BP 2078, F-06606 Antibes cedex, France; and fourth author: Virologie, UMR GD2P, IBVM, INRA, BP 81, F-33883 Villenave d'Ornon cedex, France

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Accepted for publication 20 September 2000.

A survey for viruses in rose propagated in Europe resulted in detection of only Prunus necrotic ringspot virus (PNRSV) among seven viruses screened. Four percent of cut-flower roses from different sources were infected with PNRSV. Progression of the disease under greenhouse conditions was very slow, which should make this virus easy to eradicate through sanitary selection. Comparison of the partial coat protein gene sequences for three representative rose isolates indicated that they do not form a distinct phylogenetic group and show close relations to Prunus spp. isolates. However, a comparison of the reactivity of monoclonal antibodies raised against these isolates showed that the most prevalent PNRSV serotype in rose was different from the most prevalent serotype in Prunus spp. All of the 27 rose isolates tested infected P. persica seedlings, whereas three of the four PNRSV isolates tested from Prunus spp. were poorly infectious in Rosa indica plants. These data suggest adaptation of PNRSV isolates from Prunus spp., but not from rose, to their host plants. The test methodologies developed here to evaluate PNRSV pathogenicity in Prunus spp. and rose could also help to screen for resistant genotypes.

Additional keywords: in vitro screening , woody plant.

The American Phytopathological Society, 2001