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VIEW ARTICLE   |    DOI: 10.1094/MPMI-9-0729

Identification and Map Location of TTR1, a Single Locus in Arabidopsis thaliana that Confers Tolerance to Tobacco Ringspot Nepovirus. Jian- Ming Lee. Physiological and Molecular Plant Biology Program, University of Illinois at Urbana-Champaign, Urbana, IL 61801-4723. Glen L. Hartman(2), Leslie L. Domier(2), and Andrew F. Bent(3) (1) Physiological and Molecular Plant Biology Program; (2) Crop Protection Research Unit, USDA, Agricultural Research Service, and Department of Crop Sciences; (3) Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801-4723. MPMI 9:729-735. Accepted 12 July 1996. Copyright 1996 The American Phytopathological Society.

The interaction between Arabidopsis and the nepovirus tobacco ringspot virus (TRSV) was characterized. Of 97 Arabidopsis lines tested, all were susceptible when inoculated with TRSV grape strain. Even though there was systemic spread of the virus, there was a large degree of variation in symptoms as the most sensitive lines died 10 days after inoculation, while the most tolerant lines either were symptomless or developed only mild symptoms. Four lines were selected for further study based on their differential reactions to TRSV. Infected plants of line Col-0 and CoI-0 gll flowered and produced seeds like noninfected plants, while those of lines Estland and H55 died before producing seeds. Symptoms appeared on sensitive plants approximately 5 to 6 days after inoculation. Serological studies indicated that in mechanically inoculated seedlings, the virus, as measured by coat protein accumulation, developed at essentially the same rates and to the same levels in each of the four lines, demonstrating that differences in symptom development were not due to a suppression of virus accumulation. Two additional TRSV strains gave similar results when inoculated on the four lines. Genetic studies with these four Arabidopsis lines revealed segregation of a single incompletely dominant locus controlling tolerance to TRSV grape strain. We have designated this locus TTR1. By using SSLP and CAPS markers, TTR1 was mapped to chromosome V near the ngal29 marker. Seed transmission frequency of TRSV for CoI-0 and Col-0 gll was over 95% and their progeny from crosses all had seed transmission frequencies of over 83%, which made it possible to evaluate the segregation of TTRl in F2 progeny from infected F1 plants without inoculating F2 plants. Seed transmission of TRSV will be further exploited to streamline selection of individuals for fine mapping the TTRl gene. The identification of tolerant and sensitive interactions between TRSV and A. thaliana lines provides a model system for genetic and molecular analysis of plant tolerance to virus infection.