VIEW ARTICLE | DOI: 10.1094/MPMI-8-0996
Characterization of a Gene Cluster of Phytophthora cryptogea Which Codes for Elicitins, Proteins Inducing a Hypersensitive-Like Response in Tobacco. Franck Panabieres . Station de Botanique et de Pathologie vegetale, INRA, BP 2078, F-06600, Antibes, France. Antoine Marais (1), Jo-Yanne Le Berre (1), Isabelle Penot (1), Didier Fournier (2), and Pierre Ricci (1). (1) Station de Botanique et de Pathologie vegetale, INRA, BP 2078, F-06600, Antibes, France; and (2) Laboratoire d'Entomologie, Universite Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France. MPMI 8:996-1003. Accepted 12 July 1995. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1995.
Additional Keywords: avirulence factors, elicitors, multigene family, Phytophthora cryptogea
Elicitins, proteinaceous elicitors secreted by Phytophthora spp., act as inducers of a hypersensitive-like response in tobacco during incompatible interactions. We have isolated and cloned sequences encoding cryptogein and related isoforms from P. cryptogea that belong to the elicitin family. The isolation of a genomic clone led to the characterization of four clustered genes. Two of these genes encode distinct elicitins, and two genes would encode, if expressed, a class of highly acidic elicitins which had not been observed so far. Northern blots indicate that elicitin genes are expressed in the fungus grown in vitro, though at different levels. Southern hybridization revealed that elicitins are encoded by a multigene family in several other species of Phytophthora. Moreover, isolates of Phytophthora parasitica van nicotianae, pathogenic to tobacco, which do not produce elicitins, possess several elicitin-encoding genes. Involvement of elicitins in plant-pathogen interactions is discussed.