The RFLP Molecular Marker Closely Linked to the Supernodulation Locus of Soybean Contains Three Inserts. Deborah Landau Ellis. Plant Molecular Genetics, Institute of Agriculture, and Center for Legume Research, The University of Tennessee, Knoxville 37901-1071 U.S.A. Peter M. Gresshoff. Plant Molecular Genetics, Institute of Agriculture, and Center for Legume Research, The University of Tennessee, Knoxville 37901-1071 U.S.A. MPMI 7:432-433. Accepted 18 February 1994 Copyright 1994 The American Phytopathological Society .

The pA-132 probe, linked to the supernodulation (nts) locus of soybean, was generated as a random genomic Pstl insert. The insert, however, was not unique, and three Pstl fragments were detected in the probe. To clarify whether the three fragments were contiguous, we analyzed a genomic lambda clone as well as the segregation of the three inserts in an F population.