VIEW ARTICLE | DOI: 10.1094/MPMI-7-0482
Abnormal Bacteroid Development in Nodules Induced by a Glucosamine Synthase Mutant of Rhizobium ieguminosarum . C. Marie. John Innes Institute, John Innes Centre, Colney Lane, Norwich NR4 7UH, U.K; K. A. Plaskitt and J. A. Downie.
John Innes Institute, John Innes Centre, Colney Lane, Norwich NR4 7UH, U.K. MPMI 7:482-487. Accepted 25 March 1994. Copyright 1994 The American Phytopathological Society.
Additional Keywords: Pisum salivum
Mutation of the chromosomal gene (glmS) encoding glucosamine synthase in Rhizobium leguminosarum biovar viciae results in a mutant that can induce nodules on peas, but with greatly reduced level of symbiotic nitrogen fixation. Electron microscopy of the nodules revealed that infection and release of the glmS mutant from infection threads was normal. However, the subsequent development of bacteroids was abnormal; bacteroids in the mature zone of the nodule were much larger than controls, were abnormally shaped and highly vacuolated, and underwent rapid senescence. It is proposed that expression of nodM (also encoding a glucosamine synthase), present on the symbiotic plasmid, enabled the mutant to grow in the rhizosphere and within infection threads, hut when the bacteria were released from infection threads, the nod genes (including nodM) were no longer expressed, resulting in glucosamine limitation of the bacteroids. Similarly, glucosamine limitation in free-living cultures caused a significant reduction in the amount of cell wall lipopolysaccharide and in qualitative changes to the lipo-polysaccharide, as revealed by probing with monoclonal antibodies targeted against lipopolysaccharide epitopes.