VIEW ARTICLE | DOI: 10.1094/MPMI-7-0419
DNA Sequence and Expression Analysis
of Root-Knot Nematode-Elicited Giant Cell Transcripts. David Bird. Department of Nematology and Graduate Genetics Group, University of California, Riverside 92521 U.S.A. Mark A. Wilson. Department of Nematology and Graduate Genetics Group, University of California, Riverside 92521 U.S.A. MPMI 7:419-424. Accepted 14 February 1994. Copyright 1994 The American Phytopathological Society.
Fifty-eight cDNA clones isolated from a library of transcripts exhibiting up regulation in tomato root giant cells induced by infection with the parasitic nematode Meloi-dogyne incognita were characterized. A survey of plant tissues identified 31 transcripts present in tissues other than root, including actively dividing and expanding tissues and mature leaf tissues. The identities of approximately 20% of the giant cell transcripts were inferred from DNA sequence data; they include sequences encoding a plasmalemma proton ATPase, a putative Myb-type transcription factor, and the largest subunit of RNA polymerase II.