Cytoskeletal Components in the Arbuscular Mycorrhizal Fungus Glomus mosseae . Helena Astrom. Department of Botany, University of Helsinki, P.O. Box 7 (Unioninkatu 44), 00014 University of Helsinki, Helsinki, Finland. Manuela Giovannetti(2), and Marjatta Raudaskoski(1). (1) Department of Botany, University of Helsinki, P.O. Box 7 (Unioninkatu 44), 00014 University of Helsinki, Helsinki, Finland, and (2) Istituto di Microbiologia Agraria, Centro di Studio per !a Microbiologia del Suolo, Via del Borghetto 80, 56124 Pisa, Italy. MPMI 7:309-312. Accepted 30 November 1993. Copyright 1994 The American Phytopathological Society.

In Glomus mosseae microlubules and microfilaments were visualized by indirect immunofluorescence microscopy in hyphae quick-frozen, freeze-substituted, and treated with cell-wall-degrading enzymes. Microlubules were distinguished in both the cortical and the central parts of hyphae, while microfilaments were revealed only in the cortical part. In immunoblotting, tubulin and actin were detected in an extract from hyphae elicited by host plants.