RFLP Markers Linked to the Durable Stem Rust Resistance Gene Rpg1 in Barley. A Kilian. Department of Crop and Soil Sciences and Genetics and Cell Biology, Washington State University, Pullman 99164 U.S.A. B. J. Steffenson(2), M. A. Saghai Maroof(3), and A. Kleinhofs(1). (1)Department of Crop and Soil Sciences and Genetics and Cell Biology, Washington State University, Pullman 99164 U.S.A.; (2)Department of Plant Pathology, North Dakota State University, Fargo, 58015 U.S.A.; (3)Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 U.S.A. MPMI 7:298-301. Accepted 30 November 1993. Copyright 1994 The American Phytopathological Society.

The gene, Rpgl, conferring stable resistance in barley to the wheat stem rust pathogen (Puccinia graminis f. sp. tritici)was mapped using two doubled haploid populations. Rpgl mapped to the extreme subteleomeric region of barley chromosome IP 0.3 and 1.1 cM proximal from the molecular markers ABG704 and plastocyanin (Plc), respectively, and 2.2 cM distal from MWG036B. The closest marker, ABG704, was sequenced and PCR-based markers were developed.