VIEW ARTICLE | DOI: 10.1094/MPMI-6-467
Cloning and Targeted Gene Disruption of XYL1, a Beta1,4-Xylanase Gene from the Maize Pathogen Cochliobolus carbonum. Patricia C. Apel. DOE-Plant Research Laboratory, Michigan State University, East Lansing 48824-1312 U.S.A. Daniel G. Panaccione, Frank R. Holden, and Jonathan D. Walton. DOE-Plant Research Laboratory, Michigan State University, East Lansing 48824-1312 U.S.A. MPMI 6:467-473. Accepted 23 March 1993. Copyright 1993 The American Phytopathological Society.
Additional Keywords: cell wall degrading enzyme, homologous recombination, Helminthosporium.
The gene, XYL1, encoding the major extracellular endo-Beta1,4-xylanase from the maize pathogen Cochliobolus carbonum was cloned using a synthetic, degenerate oligonucleotide based on a tryptic fragment from the purified enzyme. The deduced product of XYL1 has a Mr of 20,869 and a predicted pI of 9.1, in good agreement with the measured Mr and pI of the purified enzyme. The XYL1 product has strong amino acid identity to seven endo-Beta1,4-xylanases from six prokaryotes but no obvious similarity to 10 other prokaryotic endoxylanases or a yeast endoxylanase. An internal fragment of the gene was used to create a specific xylanase mutant by transformation-mediated gene disruption via homologous recombination. Total extracellular xylanase activity in the mutant was reduced by 85-94%. When analyzed by cation exchange HPLC, culture filtrates of the mutant and wild type had identical protein profiles, but the mutant lacked the major peak of UV absorption corresponding to the major xylanase activity. Xylanase II activity was also missing in the mutant, but xylanase III activity was still present. The XYL1 mutant grew as well as the wild type on sucrose, on corn cell walls, and on xylan. The pathogenicity of the mutant was indistinguishable from the wild type, indicating that XYL1 is not required for pathogenicity.