High Resistance to Cucumber Mosaic Virus Conferred by Satellite RNA and Coat Protein in Transgenic Commercial Tobacco Cultivar G-140. Y. Yie. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P.R. China. F. Zhao, S. Z. Zhao, Y. Z. Liu, Y. L. Liu, and Po Tien. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P.R. China.. MPMI 5:460-465. Accepted 25 June 1992. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1992.

A chimeric vector was constructed to express cucumber mosaic virus (CMV) satellite (Sat) RNA and coat protein (CP). Transgenic lines of tobacco cultivar G-140 expressing CP and Sat-RNA were obtained; these lines had high resistance to CMV. Fifty to 70% of the transgenic plants were symptomless 90 days after inoculation with 25-50 μg/ml of CMV. Resistance was about twice that conferred by the Sat-RNA or the CP gene alone in transformed plants.