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VIEW ARTICLE   |    DOI: 10.1094/MPMI-5-096


Proteins Associated with Root-Hair Deformation and Nodule Initiation in Vigna unguiculata. Andrea Krause. Laboratoire de Biologie Moléculaire des Plantes Supérieures, Université de Genève, Switzerland. William J. Broughton. Laboratoire de Biologie Moléculaire des Plantes Supérieures, Université de Genève, Switzerland.. MPMI 5:96-103. Accepted 25 September 1991. Copyright 1992 The American Phytopathological Society.


Developmental changes in the synthesis of root-hair proteins were followed in the symbiosis between the promiscuous legume Vigna unguiculata and the broad host-range Rhizobium sp. NGR234. Comparison of the two-dimensional electrophoresis patterns of proteins isolated from root hairs inoculated with wild-type or hair-deformation minus mutants revealed 12 symbiosis-specific proteins. Synthesis of three of these proteins was repressed 4 days after inoculation with Rhizobium. The remaining nine proteins were induced by Rhizobium 1, 2, or 4 days after inoculation. As three of these (15-, 31-, and 44-kDa hadulins) were specifically and transiently expressed in root hairs during the deformation process, we have named them hadulins (hair-deformation specific proteins). Five proteins (including 15- and 31-kDa hadulins) were first observed 24 hr after inoculation. Of these, only the 15-kDa hadulin was not induced by R. fredii USDA257S1 Nod+, Fix+ on V. unguiculata). Two days after inoculation, three additional proteins became apparent, while another (44-kDa hadulin) appeared on day 4. All 12 proteins seem to be associated with root-hair deformation and nodule development.

Additional Keywords: developmental changes, early nodulins, two-dimensional gels.