VIEW ARTICLE | DOI: 10.1094/MPMI-3-358
Root-Associated Enterobacter and Klebsiella in Poa pratensis: Characterization of an Iron-Scavenging System and a Substance Stimulating Root Hair Production. Kielo Haahtela. (1)Department of General Microbiology, University of Helsinki, SF-00300 Helsinki, Finland . Reijo Rönkkö(1), Tuula Laakso(1), Peter H. Williams(2), and Timo K. Korhonen(1). (1)Department of General Microbiology, University of Helsinki, SF-00300 Helsinki, Finland; (2)Department of Genetics, University of Leicester, Leicester LE1 7RH, U.K.. MPMI 3:358-365. Accepted 7 June 1990. Copyright 1990 The American Phytopathological Society.
Additional Keywords: associative N2-fixers, root morphology.
Forty strains of Enterobacter agglomerans, E. aerogenes, E. cloacae, Klebsiella pneumoniae, and K. terrigena isolated from plants or humans were analyzed for iron-scavenging systems, plant growth-promoting effects on grasses, and production of auxins and related indole compounds. Enterochelin was produced by all isolates of Klebsiella, none of which produced aerobactin. None of the isolates of Enterobacter from plants produced enterochelin or aerobactin, whereas isolates from humans produced both siderophore types. Inoculation with each enterobacterial isolate significantly increased the number of root hairs of Poa pratensis, with no significant difference between bacteria from plants or humans. Cellfree ethyl acetate extracts were tested on newly germinated roots of P. pratensis. Extracts obtained at pH 7.0 significantly increased the number of root hairs, whereas extracts obtained at pH 2.8 increased production of root hairs only in few plants. A bioactive compound causing increased production of root hairs was isolated and characterized from the culture supernatant of a strain of E. agglomerans. Gas chromatography-mass spectrometry (GC-MS) analysis of this compound proved that the bioactive substance was an auxin, indole-3-acetic acid. Thin-layer chromatographic analysis of the neutral extracts showed that the enterobacterial isolates produced at least 10 indole compounds from which eight were identified by GC-MS. Slight differences in spectra of indole compounds were observed between bacterial isolates from plants and humans, but indole-3-acetic acid was detected in 88% of the enterobacterial isolates.