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Tomato Whole Genome Transcriptional Response to Tetranychus urticae Identifies Divergence of Spider Mite-Induced Responses Between Tomato and Arabidopsis

March 2015 , Volume 28 , Number  3
Pages  343 - 361

Catherine Martel,1 Vladimir Zhurov,1 Marie Navarro,1,2 Manuel Martinez,3 Marc Cazaux,1,2 Philippe Auger,4 Alain Migeon,4 M. Estrella Santamaria,1,3 Nicky Wybouw,5 Isabel Diaz,3 Thomas Van Leeuwen,5,6 Maria Navajas,4 Miodrag Grbic,1,2 and Vojislava Grbic1,2

1Department of Biology, The University of Western Ontario, London, ON, Canada N6A5B7; 2Instituto de Ciencias de la Vid y el Vino, 26006 Logrono, Spain; 3Centro de Biotecnologia y Genomica de Plantas, Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28223 Madrid, Spain; 4INRA, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), 34998 Montferrier-sur-Lez Cedex, France; 5Department of Crop Protection, Ghent University, B-9000, Ghent, Belgium; 6Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, 1098 XH, Amsterdam, The Netherlands

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Accepted 22 December 2014.

The two-spotted spider mite Tetranychus urticae is one of the most significant mite pests in agriculture, feeding on more than 1,100 plant hosts, including model plants Arabidopsis thaliana and tomato, Solanum lycopersicum. Here, we describe timecourse tomato transcriptional responses to spider mite feeding and compare them with Arabidopsis in order to determine conserved and divergent defense responses to this pest. To refine the involvement of jasmonic acid (JA) in mite-induced responses and to improve tomato Gene Ontology annotations, we analyzed transcriptional changes in the tomato JA-signaling mutant defenseless1 (def-1) upon JA treatment and spider mite herbivory. Overlay of differentially expressed genes (DEG) identified in def-1 onto those from the timecourse experiment established that JA controls expression of the majority of genes differentially regulated by herbivory. Comparison of defense responses between tomato and Arabidopsis highlighted 96 orthologous genes (of 2,133 DEG) that were recruited for defense against spider mites in both species. These genes, involved in biosynthesis of JA, phenylpropanoids, flavonoids, and terpenoids, represent the conserved core of induced defenses. The remaining tomato DEG support the establishment of tomato-specific defenses, indicating profound divergence of spider mite–induced responses between tomato and Arabidopsis.

© 2015 The American Phytopathological Society