María Inés Siri,1
Christian Boucher,2 and
María Julia Pianzzola1
1Cátedra de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Av. Gral. Flores 2124, CP11800, Montevideo, Uruguay; 2Laboratoire des Interactions Plantes Micro-organismes (LIPM), UMR CNRS-INRA 2594/441, F-31320 Castanet Tolosan, France
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Accepted 19 February 2014.
This study provides insights into the pathogenesis of Ralstonia solanacearum, in particular with regards to strains belonging to phylotype IIB, sequevar 1 (IIB-1) and their interaction with potato, its natural host. We performed a comparative genomic analysis among IIB-1 R. solanacearum strains with different levels of virulence in order to identify candidate virulence genes. With this approach, we identified a 33.7-kb deletion in a strain showing reduced virulence on potato. This region contains a cluster of six genes putatively involved in type IV pili (Tfp) biogenesis. Functional analysis suggests that these proteins contribute to several Tfp-related functions such as twitching motility and biofilm formation. In addition, this genetic cluster was found to contribute to early bacterial wilt pathogenesis and colonization fitness of potato roots.
© 2014 The American Phytopathological Society