Link to home

Fine Tuning of Reactive Oxygen Species Homeostasis Regulates Primed Immune Responses in Arabidopsis

November 2013 , Volume 26 , Number  11
Pages  1,334 - 1,344

Victoria Pastor,1 Estrella Luna,2 Jurriaan Ton,2 Miguel Cerezo,1 Pilar García-Agustín,1 and Victor Flors1

1Plant Physiology Section, Department of CAMN, Univesitat Jaume I, 12071, Castellón, Spain; 2Department of Animal and Plant Sciences, Alfred Denny Building, The University of Sheffield, Sheffield, S10 2TN, U.K.

Go to article:
Accepted 4 July 2013.

Selected stimuli can prime the plant immune system for a faster and stronger defense reaction to pathogen attack. Pretreatment of Arabidopsis with the chemical agent β-aminobutyric acid (BABA) augmented H2O2 and callose production after induction with the pathogen-associated molecular pattern (PAMP) chitosan, or inoculation with the necrotrophic fungus Plectosphaerella cucumerina. However, BABA failed to prime H2O2 and callose production after challenge with the bacterial PAMP Flg22. Analysis of Arabidopsis mutants in reactive oxygen species (ROS) production (rbohD) or ROS scavenging (pad2, vtc1, and cat2) suggested a regulatory role for ROS homeostasis in priming of chitosan- and P. cucumerina-inducible callose and ROS. Moreover, rbohD and pad2 were both impaired in BABA-induced resistance against P. cucumerina. Gene expression analysis revealed direct induction of NADPH/respiratory burst oxidase protein D (RBOHD), γ-glutamylcysteine synthetase 1 (GSH1), and vitamin C defective 1 (VTC1) genes after BABA treatment. Conversely, ascorbate peroxidase 1 (APX1) transcription was repressed by BABA after challenge with chitosan or P. cucumerina, probably to provide a more oxidized environment in the cell and facilitate augmented ROS accumulation. Measuring ratios between reduced and oxidized glutathione confirmed that augmented defense expression in primed plants is associated with a more oxidized cellular status. Together, our data indicate that an altered ROS equilibrium is required for augmented defense expression in primed plants.

© 2013 The American Phytopathological Society