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The Absence of Eukaryotic Initiation Factor eIF(iso)4E Affects the Systemic Spread of a Tobacco etch virus Isolate in Arabidopsis thaliana

April 2013 , Volume 26 , Number  4
Pages  461 - 470

Carlos A. Contreras-Paredes,1 Laura Silva-Rosales,2 José-Antonio Daròs,3 Naholi D. Alejandri-Ramírez,1 and Tzvetanka D. Dinkova1

1Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, 04510, México, D. F., México; 2Departamento de Ingeniería Genética, Cinvestav-Irapuato, Km 9.6 Lib. Nte. Carr. Irapuato-León, Irapuato, Gto. 36821 México; 3Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia), Avenida de los Naranjos, 46022 Valencia, Spain

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Accepted 4 December 2012.

Translation initiation factor eIF4E exerts an important role during infection of viral species in the family Potyviridae. Particularly, a eIF(iso)4E family member is required for Arabidopsis thaliana susceptibility to Turnip mosaic virus, Lettuce mosaic virus, and Tobacco etch virus (TEV). In addition, a resistance mechanism named restriction of TEV movement (RTM) in A. thaliana controls the systemic spread of TEV in Col-0 ecotype. Here, we describe that TEV-TAMPS, a Mexican isolate, overcomes the RTM resistance mechanism reported for TEV-7DA infection of the Col-0 ecotype but depends on eIF(iso)4E for its systemic spread. To understand at which level eIF(iso)4E participates in A. thaliana TEV-TAMPS infection, the viral RNA replication and translation were measured. The absence or overexpression of eIF(iso)4E did not affect viral translation, and replication was still observed in the absence of eIF(iso)4E. However, the TEV-TAMPS systemic spread was completely abolished in the null mutant. The viral protein genome-linked (VPg) precursor NIa was found in coimmunoprecipitated complexes with both, eIF(iso)4E and eIF4E. However, the viral coat protein (CP) was only present in the eIF(iso)4E complexes. Since both the VPg and the CP proteins are needed for systemic spread, we propose a role of A. thaliana eIF(iso)4E in the movement of TEV-TAMPS within this host.

© 2013 The American Phytopathological Society