Link to home

The Requirement of Multiple Defense Genes in Soybean Rsv1–Mediated Extreme Resistance to Soybean mosaic virus

October 2012 , Volume 25 , Number  10
Pages  1,307 - 1,313

Chunquan Zhang, Sehiza Grosic, Steven A. Whitham, and John H. Hill

Department of Plant Pathology & Microbiology, Iowa State University, Ames 50011, U.S.A.

Go to article:
Accepted 7 June 2012.

Soybean mosaic virus (SMV) is a major viral pathogen of soybean. Among the three SMV resistance genes, Rsv1 mediates extreme resistance (ER) against most SMV strains, including the β-glucuronidase-tagged G2 isolate that was previously used in studies of Rsv1. Using virus-induced gene silencing (VIGS), we screened 82 VIGS constructs to identify genes that play a role in Rsv1-mediated ER to SMV infection. The target genes included putative Rsv1 candidate genes, soybean orthologs to known defense-signaling genes, and 62 WRKY transcription factors. We identified eight VIGS constructs that compromised Rsv1-mediated resistance when the target genes were silenced, including GmEDR1, GmEDS1, GmHSP90, GmJAR1, GmPAD4, and two WRKY transcription factors. Together, our results provide new insight into the soybean signaling network required for ER against SMV.

© 2012 The American Phytopathological Society