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Pathogen-Induced Accumulation of an Ellagitannin Elicits Plant Defense Response

November 2012 , Volume 25 , Number  11
Pages  1,430 - 1,439

Alicia Mamaní,1 María Paula Filippone,2 Carlos Grellet,1 Björn Welin,2 Atilio Pedro Castagnaro,2 and Juan Carlos Díaz Ricci1

1Instituto Superior de Investigaciones Biológicas (INSIBIO; CONICET-UNT) e Instituto de Química Biológica “Dr. Bernabé Bloj”, Universidad Nacional de Tucumán, Chacabuco 461, 4000, San Miguel de Tucumán, Argentina; 2Sección Biotecnología de la Estación Experimental Agroindustrial Obispo Colombres (EEAOC)-Unidad Asociada al INSIBIO, Av. William Cross 3150, Las Talitas, 4101, Tucumán, Argentina

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Accepted 3 July 2012.

In an incompatible interaction between Colletotrichum fragariae and strawberry plants, the accumulation of phenolic compounds in plant leaves was observed. A particularly abundant penta-esterified ellagitannin that accumulated in response to pathogen attack was identified as 1-0-galloyl-2,3;4,6-bis-hexahydroxydiphenoyl-β-D-glucopyranose (HeT) by mass spectroscopy and nuclear magnetic resonance. Foliar application of purified HeT prior to inoculation with a virulent pathogen was shown to increase resistance toward C. acutatum in strawberry plants and to Xanthomonas citri subsp. citri in lemon plants. The induced resistance in strawberry was associated with a rapid oxidative burst, callose deposition, a transient increase of salicylic acid in phloem, and induction of gene expression responsive to salicylic acid. Results obtained suggested that HeT could be a common plant defense response molecule capable of inducing pathogen resistance in different plant species.

© 2012 The American Phytopathological Society