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Germination Stimulants of Phelipanche ramosa in the Rhizosphere of Brassica napus Are Derived from the Glucosinolate Pathway

July 2012 , Volume 25 , Number  7
Pages  993 - 1,004

Bathilde Auger,1 Jean-Bernard Pouvreau,1 Karinne Pouponneau,2 Kaori Yoneyama,3 Grégory Montiel,1 Bruno Le Bizec,2 Koichi Yoneyama,3 Philippe Delavault,1 Régine Delourme,4 and Philippe Simier1

1LUNAM Université, Laboratoire de Biologie et Pathologie Végétales, SFR 4207 QUASAV, UFR Sciences et Techniques, F-44322 Nantes, France; 2LUNAM Université, Oniris, LABERCA, F-44307 Nantes, France; 3Utsunomiya University, Weed Science Center, Utsunomiya 321-8505, Japan; 4INRA, UMR1349 IGEPP, F-35653 Le Rheu, France

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Accepted 8 March 2012.

Phelipanche ramosa is a major parasitic weed of Brassica napus. The first step in a host-parasitic plant interaction is stimulation of parasite seed germination by compounds released from host roots. However, germination stimulants produced by B. napus have not been identified yet. In this study, we characterized the germination stimulants that accumulate in B. napus roots and are released into the rhizosphere. Eight glucosinolate-breakdown products were identified and quantified in B. napus roots by gas chromatography–mass spectrometry. Two (3-phenylpropanenitrile and 2-phenylethyl isothiocyanate [2-PEITC]) were identified in the B. napus rhizosphere. Among glucosinolate-breakdown products, P. ramosa germination was strongly and specifically triggered by isothiocyanates, indicating that 2-PEITC, in particular, plays a key role in the B. napusP. ramosa interaction. Known strigolactones were not detected by ultraperformance liquid chromatography–tandem mass spectrometry, and seed of Phelipanche and Orobanche spp. that respond to strigolactones but not to isothiocyanates did not germinate in the rhizosphere of B. napus. Furthermore, both wild-type and strigolactone biosynthesis mutants of Arabidopsis thaliana Atccd7 and Atccd8 induced similar levels of P. ramosa seed germination, suggesting that compounds other than strigolactone function as germination stimulants for P. ramosa in other Brassicaceae spp. Our results open perspectives on the high adaptation potential of root-parasitic plants under host-driven selection pressures.

This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2012.