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Rapid Mobilization of Membrane Lipids in Wheat Leaf Sheaths During Incompatible Interactions with Hessian Fly

July 2012 , Volume 25 , Number  7
Pages  920 - 930

Lieceng Zhu,1 Xuming Liu,2 Haiyan Wang,3 Chitvan Khajuria,2 John C. Reese,2 R. Jeff Whitworth,2 Ruth Welti,4 and Ming-Shun Chen2,5

1Department of Biological Science, Fayetteville State University, Fayetteville, NC 28301, U.S.A.; 2Department of Entomology, 3Department of Statistics, and 4Division of Biology, Kansas State University, Manhattan 66506, U.S.A.; 5United States Department of Agriculture–Agricultural Research Service, Hard Winter Wheat Genetics Research Unit, 4008 Throckmorton Hall, Kansas State University, Manhattan 66506, U.S.A.

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Accepted 28 March 2012.

Hessian fly (HF) is a biotrophic insect that interacts with wheat on a gene-for-gene basis. We profiled changes in membrane lipids in two isogenic wheat lines: a susceptible line and its backcrossed offspring containing the resistance gene H13. Our results revealed a 32 to 45% reduction in total concentrations of 129 lipid species in resistant plants during incompatible interactions within 24 h after HF attack. A smaller and delayed response was observed in susceptible plants during compatible interactions. Microarray and real-time polymerase chain reaction analyses of 168 lipid-metabolism-related transcripts revealed that the abundance of many of these transcripts increased rapidly in resistant plants after HF attack but did not change in susceptible plants. In association with the rapid mobilization of membrane lipids, the concentrations of some fatty acids and 12-oxo-phytodienoic acid (OPDA) increased specifically in resistant plants. Exogenous application of OPDA increased mortality of HF larvae significantly. Collectively, our data, along with previously published results, indicate that the lipids were mobilized through lipolysis, producing free fatty acids, which were likely further converted into oxylipins and other defense molecules. Our results suggest that rapid mobilization of membrane lipids constitutes an important step for wheat to defend against HF attack.

This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2012.