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Infection Dynamics in Viral Spread and Interference Under the Synergism Between Cucumber mosaic virus and Turnip mosaic virus

January 2012 , Volume 25 , Number  1
Pages  18 - 27

Minoru Takeshita,1 Emiko Koizumi,1 Makiko Noguchi,1 Kae Sueda,2 Hanako Shimura,2 Noriko Ishikawa,3 Hideyuki Matsuura,2 Kazusato Ohshima,4 Tomohide Natsuaki,3 Shigeru Kuwata,5 Naruto Furuya,1 Kenichi Tsuchiya,1 and Chikara Masuta2

1Laboratory of Plant Pathology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; 2Graduate School of Agriculture, Hokkaido University, Kita-ku kita 9, Nishi 9, Sapporo 060-8589, Japan; 3Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya 321-8505, Japan; 4Faculty of Agriculture, Saga University, Saga 840-8502, Japan; 5School of Agriculture, Meiji University, Tama-ku, Kawasaki 214-8571, Japan

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Accepted 25 August 2011.

Mixed infection of Cucumber mosaic virus (CMV) and Turnip mosaic virus (TuMV) induced more severe symptoms on Nicotiana benthamiana than single infection. To dissect the relationships between spatial infection patterns and the 2b protein (2b) of CMV in single or mixed infections, the CMV vectors expressing enhanced green fluorescent or Discosoma sp. red fluorescent proteins (EGFP [EG] or DsRed2 [Ds], respectively were constructed from the same wild-type CMV-Y and used for inoculation onto N. benthamiana. CMV2-A1 vector (C2-A1 [A1]) has a functional 2b while CMV-H1 vector (C2-H1 [H1]) is 2b deficient. As we expected from the 2b function as an RNA silencing suppressor (RSS), in a single infection, A1Ds retained a high level of accumulation at initial infection sites and showed extensive fluorescence in upper, noninoculated leaves, whereas H1Ds disappeared rapidly at initial infection sites and could not spread efficiently in upper, noninoculated leaf tissues. In various mixed infections, we found two phenomena providing novel insights into the relationships among RSS, viral synergism, and interference. First, H1Ds could not spread efficiently from vasculature into nonvascular tissues with or without TuMV, suggesting that RNA silencing was not involved in CMV unloading from vasculature. These results indicated that 2b could promote CMV to unload from vasculature into nonvascular tissues, and that this 2b function might be independent of its RSS activity. Second, we detected spatial interference (local interference) between A1Ds and A1EG in mixed infection with TuMV, between A1Ds (or H1Ds) and TuMV, and between H1Ds and H1EG. This observation suggested that local interference between two viruses was established even in the synergism between CMV and TuMV and, again, RNA silencing did not seem to contribute greatly to this phenomenon.

© 2012 The American Phytopathological Society