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The Botrytis cinerea Reg1 Protein, a Putative Transcriptional Regulator, Is Required for Pathogenicity, Conidiogenesis, and the Production of Secondary Metabolites

September 2011 , Volume 24 , Number  9
Pages  1,074 - 1,085

Caroline B. Michielse,1 Matthias Becker,1 Jens Heller,1 Javier Moraga,2 Isidro G. Collado,2 and Paul Tudzynski1

1Institute of Biology and Biotechnology of Plants, Westfälische Wilhelms-University Münster, Schlossgarten 3, D-48149 Münster, Germany; 2Departamento de Quimica Organica, Facultad de Ciencias, Universidad de Cadiz, Puerto Real, Spain

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Accepted 11 May 2011.

Botrytis cinerea, which causes gray-mold rot, attacks a wide range of plant species. To understand the infection process, the role of a putative transcriptional regulator, BcReg1 (regulator 1), in pathogenicity was studied. This transcriptional regulator shows similarity to the morphological switch regulators Candida albicans Wor1 and Histoplasma capsulatum Ryp1. Gene knock-out and complementation studies revealed that bcreg1 is required for pathogenicity. The bcreg1 mutant is able to penetrate plant tissue but is not able to cause necrotic lesions. In addition, the mutant is blocked in conidia formation and does not produce detectable levels of the sesquiterpene botrydial and the polyketide botcinic acid. Based on transcript expression levels, it can be concluded that bcreg1 is a downstream target of two mitogen-activated protein kinases, BcSak1 and Bmp3.

© 2011 The American Phytopathological Society