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Identification and Characterization of 2′-Deoxyuridine from the Supernatant of Conidial Suspensions of Rice Blast Fungus as an Infection-Promoting Factor in Rice Plants

May 2011 , Volume 24 , Number  5
Pages  519 - 532

Sugihiro Ando,1 Yuko Sato,1 Hideyuki Shigemori,2 Takafumi Shimizu,3 Kazunori Okada,3 Hisakazu Yamane,3 Yusuke Jikumaru,4 Yuji Kamiya,4 Kosumi Yamada,2 Chiharu Akimoto-Tomiyama,1 Shigeru Tanabe,1 Yoko Nishizawa,1 and Eiichi Minami1

1Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan; 2Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; 3Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; 4RIKEN, Plant Science Center, Tsurumi-ku, Yokohama 230-0045, Japan


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Accepted 15 December 2010.

We previously detected infection-promoting activity in the supernatant of the conidial suspension (SCS) of the rice blast fungus. In the present study, a molecule carrying the activity was purified and identified as 2′-deoxyuridine (dU). The infection-promoting activity of dU was strictly dependent on its chemical structure and displayed characteristics consistent with those of the SCS. Notably, the activity of dU was exclusively detected during interactions between rice and virulent isolates of the fungus, the number of susceptible lesions in leaf blades was increased by dU, and nonhost resistance in rice plants was not affected by treatment with dU. In addition, the expression of pathogensis-related genes, accumulation of H2O2, and production of phytoalexins in rice in response to inoculation with virulent fungal isolates was not suppressed by dU. The infection-promoting activity of dU was not accompanied by elevated levels of endogenous abscissic acid, which is known to modify plant-pathogen interactions, and was not detected in interactions between oat plants and a virulent oat blast fungus isolate. Taken together, these results demonstrate that dU is a novel infection-promoting factor that acts specifically during compatible interactions between rice plants and rice blast fungus in a mode distinct from that of toxins and suppressors.



This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2011.