Link to home

Activation of a Lotus japonicus Subtilase Gene During Arbuscular Mycorrhiza Is Dependent on the Common Symbiosis Genes and Two cis-Active Promoter Regions

June 2011 , Volume 24 , Number  6
Pages  662 - 670

Naoya Takeda,1 Kristina Haage,1 Shusei Sato,2 Satoshi Tabata,2 and Martin Parniske1

1University of Munich (LMU), Biology, Genetics, Großhaderner Str. 2-4, 82152 Martinsried, Germany; 2Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan

Go to article:
Accepted 14 January 2011.

The subtilisin-like serine protease SbtM1 is strongly and specifically induced during arbuscular mycorrhiza (AM) symbiosis in Lotus japonicus. Another subtilase gene, SbtS, is induced during early stages of nodulation and AM. Transcript profiling in plant symbiosis mutants revealed that the AM-induced expression of SbtM1 and the gene family members SbtM3 and SbtM4 is dependent on the common symbiosis pathway, whereas an independent pathway contributes to the activation of SbtS. We used the specific spatial expression patterns of SbtM1 promoter β-D-glucuronidase (GUS) fusions to isolate cis elements that confer AM responsiveness. A promoter deletion and substitution analysis defined two cis regions (region I and II) in the SbtM1 promoter necessary for AM-induced GUS activity. 35S minimal promoter fusions revealed that either of the two regions is sufficient for AM responsiveness when tested in tandem repeat arrangement. Sequence-related regions were found in the promoters of AM-induced subtilase genes in Medicago truncatula and rice, consistent with an ancient origin of these elements predating the divergence of the angiosperms.

© 2011 The American Phytopathological Society