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The Helper Component Proteinase Cistron of Potato virus Y Induces Hypersensitivity and Resistance in Potato Genotypes Carrying Dominant Resistance Genes on Chromosome IV

July 2011 , Volume 24 , Number  7
Pages  787 - 797

Benoît Moury,1 Bernard Caromel,2 Elisabeth Johansen,3 Vincent Simon,1 Laura Chauvin,4 Emmanuel Jacquot,5 Camille Kerlan,5 and Véronique Lefebvre2

1INRA, UR407 Pathologie Végétale, F-84140 Montfavet, France; 2INRA, UR1052 Génétique et Amélioration des Fruits et Légumes, F-84140 Montfavet, France; 3Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Denmark; 4UMR INRA-Agrocampus Ouest-Université de Rennes 1, Amélioration des Plantes et Biotechnologies Végétales, Domaine de Kéraïber, F-29260 Ploudaniel, France; 5INRA-Agrocampus Ouest-Université Rennes 1, UMR1099 BiO3P (Biology of Organisms and Populations applied to Plant Protection), F-35653 Le Rheu, France

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Accepted 8 March 2011.

The Nctbr and Nytbr genes in Solanum tuberosum determine hypersensitive reactions, characterized by necrotic reactions and restriction of the virus systemic movement, toward isolates belonging to clade C and clade O of Potato virus Y (PVY), respectively. We describe a new resistance from S. sparsipilum which possesses the same phenotype and specificity as Nctbr and is controlled by a dominant gene designated Ncspl. Ncspl maps on potato chromosome IV close or allelic to Nytbr. The helper component proteinase (HC-Pro) cistron of PVY was shown to control necrotic reactions and resistance elicitation in plants carrying Ncspl, Nctbr, and Nytbr. However, inductions of necrosis and of resistance to the systemic virus movement in plants carrying Ncspl reside in different regions of the HC-Pro cistron. Also, genomic determinants outside the HC-Pro cistron are involved in the systemic movement of PVY after induction of necroses on inoculated leaves of plants carrying Nytbr. These results suggest that the Nytbr resistance may have been involved in the recent emergence of PVY isolates with a recombination breakpoint near the junction of HC-Pro and P3 cistrons in potato crops. Therefore, this emergence could constitute one of the rare examples of resistance breakdown by a virus which was caused by recombination instead of by successive accumulation of nucleotide substitutions.

This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2011.