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The Role of Catalase-Peroxidase Secreted by Magnaporthe oryzae During Early Infection of Rice Cells

February 2011 , Volume 24 , Number  2
Pages  163 - 171

Shigeru Tanabe,1 Naoko Ishii-Minami,1 Ken-Ichiro Saitoh,1,2 Yuko Otake,1 Hanae Kaku,3 Naoto Shibuya,3 Yoko Nishizawa,1 and Eiichi Minami1

1Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602, Japan; 2Advanced Career Development Center for Agro-Innovation, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, 183-8509, Japan; 3Department of Life Sciences, Meiji University, 1-1-1 Higashi-Mita, Tama-ku, Kawasaki, 214-8571, Japan

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Accepted 26 October 2010.

The biological role of a secretory catalase of the rice blast fungus Magnaporthe oryzae was studied. The internal amino acid sequences of the partially purified catalase in the culture filtrate enabled us to identify its encoding gene as a catalase-peroxidase gene, CPXB, among four putative genes for catalase or catalase-peroxidase in M. oryzae. Knockout of the gene drastically reduced the level of catalase activity in the culture filtrate and supernatant of conidial suspension (SCS), and increased the sensitivity to exogenously added H2O2 compared with control strains, suggesting that CPXB is the major gene encoding the secretory catalase and confers resistance to H2O2 in hyphae. In the mutant, the rate of appressoria that induced accumulation of H2O2 in epidermal cells of the leaf sheath increased and infection at early stages was delayed; however, the formation of lesions in the leaf blade was not affected compared with the control strain. These phenotypes were complimented by reintroducing the putative coding regions of CPXB driven by a constitutive promoter. These results suggest that CPXB plays a role in fungal defense against H2O2 accumulated in epidermal cells of rice at the early stage of infection but not in pathogenicity of M. oryzae.

© 2011 The American Phytopathological Society