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The Sesquiterpene Botrydial Produced by Botrytis cinerea Induces the Hypersensitive Response on Plant Tissues and Its Action Is Modulated by Salicylic Acid and Jasmonic Acid Signaling

August 2011 , Volume 24 , Number  8
Pages  888 - 896

Franco Rubén Rossi,1 Andrés Gárriz,1 María Marina,1 Fernando Matías Romero,1 María Elisa Gonzalez,1 Isidro González Collado,2 and Fernando Luis Pieckenstain1

1Instituto de Investigaciones Biotecnológicas Instituto Tecnológico de Chascomús/Universidad Nacional de General San Martín-Consejo Nacional de Investigaciones Científicas y Técnicas (IIB-INTECH/UNSAM-CONICET), Camino Circunvalación Laguna, Km. 6 CC 164 (B7130IWA) Chascomús, Argentina; 2Departamento de Química Orgánica, Universidad de Cádiz, Campus Ciencia y Tecnología, E-11510 Puerto Real, Spain

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Accepted 28 March 2011.

Botrytis cinerea, as a necrotrophic fungus, kills host tissues and feeds on the remains. This fungus is able to induce the hypersensitive response (HR) on its hosts, thus taking advantage on the host's defense machinery for generating necrotic tissues. However, the identity of HR effectors produced by B. cinerea is not clear. The aim of this work was to determine whether botrydial, a phytotoxic sesquiterpene produced by B. cinerea, is able to induce the HR on plant hosts, using Arabidopsis thaliana as a model. Botrydial induced the expression of the HR marker HSR3, callose deposition, and the accumulation of reactive oxygen species and phenolic compounds. Botrydial also induced the expression of PR1 and PDF1.2, two pathogenesis-related proteins involved in defense responses regulated by salicylic acid (SA) and jasmonic acid (JA), respectively. A. thaliana and tobacco plants defective in SA signaling were more resistant to botrydial than wild-type plants, as opposed to A. thaliana plants defective in JA signaling, which were more sensitive. It can be concluded that botrydial induces the HR on its hosts and its effects are modulated by host signaling pathways mediated by SA and JA.

© 2011 The American Phytopathological Society