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Direct Interaction Between the Rice yellow mottle virus (RYMV) VPg and the Central Domain of the Rice eIF(iso)4G1 Factor Correlates with Rice Susceptibility and RYMV Virulence

November 2010 , Volume 23 , Number  11
Pages  1,506 - 1,513

Eugénie Hébrard,1 Nils Poulicard,1 Clément Gérard,2 Oumar Traoré,3 Hui-Chen Wu,4,5 Laurence Albar,5 Denis Fargette,1 Yannick Bessin,2 and Florence Vignols5

1UMR186 Résistance des Plantes aux Bio-agresseurs, Institut de Recherche pour le Développement BP 64501, 34394 Montpellier cedex 5, France; 2UMR5048 Centre de Biochimie Structurale, 29 rue de Navacelles, 34090 Montpellier, France; 3Institut de l'Environnement et de Recherches Agricoles (INERA), 01 BP 476, Ouagadougou, Burkina Faso; 4Department of Life Science and Institute of Plant Biology, National Taiwan University, Taipei 10617, Taiwan; 5UMR5096 Génome et Développement des Plantes, Université de Perpignan via Domitia–CNRS–IRD, BP 64501, 34394 Montpellier cedex 5, France

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Accepted 11 July 2010.

The adaptation of Rice yellow mottle virus (RYMV) to recessive resistance mediated by the rymv1-2 allele has been reported as a model to study the emergence and evolution of virulent variants. The resistance and virulence factors have been identified as eukaryotic translation initiation factor eIF(iso)4G1 and viral genome–linked protein (VPg), respectively, but the molecular mechanisms involved in their interaction are still unknown. In this study, we demonstrated a direct interaction between RYMV VPg and the central domain of rice eIF(iso)4G1 both in vitro, using recombinant proteins, and in vivo, using a yeast two-hybrid assay. Insertion of the E309K mutation in eIF(iso)4G1, conferring resistance in planta, strongly diminished the interaction with avirulent VPg. The efficiency of the major virulence mutations at restoring the interaction with the resistance protein was assessed. Our results explain the prevalence of virulence mutations fixed during experimental evolution studies and are consistent with the respective viral RNA accumulation levels of avirulent and virulent isolates. Our results also explain the origin of the residual multiplication of wild-type isolates in rymv1-2–resistant plants and the role of genetic context in the poor adaptability of the S2/S3 strain. Finally, the strategies of RYMV and members of family Potyviridae to overcome recessive resistance were compared.

This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2010.