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Arabidopsis thaliana Cells: A Model to Evaluate the Virulence of Pectobacterium carotovorum

February 2010 , Volume 23 , Number  2
Pages  139 - 143

Meriam Terta,1,2 Mohamed Kettani-Halabi,1,2 Khadija Ibenyassine,1,2 Daniel Tran,1 Patrice Meimoun,1 Raja Ait M'hand,2 Hayat El-Maarouf-Bouteau,3 Florence Val,4 M. Mustapha Ennaji,2 and François Bouteau1

1LEM-EA3514--Université Paris Diderot-Paris 7, 2 place Jussieu, 75251 Paris cedex 05, France; 2LVHM--Université Hassan II Mohammedia-FSTM, Maroc; 3UR5--UPMC, 3 rue Galilée, 94200 Ivry sur Seine, France; 4UMR 1099 BIO3P INRA-Agrocampus Ouest-Université Rennes 1, France

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Accepted 8 October 2009.

Pectobacterium carotovorum are economically important plant pathogens that cause plant soft rot. These enterobacteria display high diversity world-wide. Their pathogenesis depends on production and secretion of virulence factors such as plant cell wall--degrading enzymes, type III effectors, a necrosis-inducing protein, and a secreted virulence factor from Xanthomonas spp., which are tightly regulated by quorum sensing. Pectobacterium carotovorum also present pathogen-associated molecular patterns that could participate in their pathogenicity. In this study, by using suspension cells of Arabidopsis thaliana, we correlate plant cell death and pectate lyase activities during coinfection with different P. carotovorum strains. When comparing soft rot symptoms induced on potato slices with pectate lyase activities and plant cell death observed during coculture with Arabidopsis thaliana cells, the order of strain virulence was found to be the same. Therefore, Arabidopsis thaliana cells could be an alternative tool to evaluate rapidly and efficiently the virulence of different P. carotovorum strains.

© 2010 The American Phytopathological Society