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Rpi-vnt1.1, a Tm-22 Homolog from Solanum venturii, Confers Resistance to Potato Late Blight

May 2009 , Volume 22 , Number  5
Pages  589 - 600

Simon J. Foster,1 Tae-Ho Park,1 Mathieu Pel,2 Gianinna Brigneti,1 Jadwiga Śliwka,3 Luke Jagger,1 Edwin van der Vossen,2 and Jonathan D. G. Jones1

1The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, NR4 7UH, U.K.; 2Wageningen UR Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands; 3Plant Breeding and Acclimatization Institute, Research Centre Młochów, Platanowa 19, 05-831 Młochów, Poland

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Accepted 22 January 2009.

Despite the efforts of breeders and the extensive use of fungicide control measures, late blight still remains a major threat to potato cultivation worldwide. The introduction of genetic resistance into cultivated potato is considered a valuable method to achieve durable resistance to late blight. Here, we report the identification and cloning of Rpi-vnt1.1, a previously uncharacterized late-blight resistance gene from Solanum venturii. The gene was identified by a classical genetic and physical mapping approach and encodes a coiled-coil nucleotide-binding leucine-rich repeat protein with high similarity to Tm-22 from S. lycopersicum which confers resistance against Tomato mosaic virus. Transgenic potato and tomato plants carrying Rpi-vnt1.1 were shown to be resistant to Phytophthora infestans. Of 11 P. infestans isolates tested, only isolate EC1 from Ecuador was able to overcome Rpi-vnt1.1 and cause disease on the inoculated plants. Alleles of Rpi-vnt1.1 (Rpi-vnt1.2 and Rpi-vnt1.3) that differed by only a few nucleotides were found in other late-blight-resistant accessions of S. venturii. The late blight resistance gene Rpi-phu1 from S. phureja is shown here to be identical to Rpi-vnt1.1, suggesting either that this strong resistance gene has been maintained since a common ancestor, due to selection pressure for blight resistance, or that genetic exchange between S. venturii and S. phureja has occurred at some time.

© 2009 The American Phytopathological Society