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Genome-Wide Analysis of Differentially Expressed Genes During the Early Stages of Tomato Infection by a Potyvirus

March 2009 , Volume 22 , Number  3
Pages  352 - 361

Poliane Alfenas-Zerbini,1 Ivan G. Maia,2 Regiane D. Fávaro,2 Julio C. M. Cascardo,3 Sérgio H. Brommonschenkel,1 and Francisco Murilo Zerbini1

1Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil; 2Instituto de Biociências/Dep. de Genética, Universidade Estadual Paulista, Botucatu, SP, 18618-000, Brazil; 3Dep. de Biologia e Genética Molecular, Universidade Estadual de Santa Cruz, Ilhéus, BA, 45662-000, Brazil

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Accepted 27 October 2008.

Plant responses against pathogens cause up- and downward shifts in gene expression. To identify differentially expressed genes in a plant-virus interaction, susceptible tomato plants were inoculated with the potyvirus Pepper yellow mosaic virus (PepYMV) and a subtractive library was constructed from inoculated leaves at 72 h after inoculation. Several genes were identified as upregulated, including genes involved in plant defense responses (e.g., pathogenesis-related protein 5), regulation of the cell cycle (e.g., cytokinin-repressed proteins), signal transduction (e.g., CAX-interacting protein 4, SNF1 kinase), transcriptional regulators (e.g., WRKY and SCARECROW transcription factors), stress response proteins (e.g., Hsp90, DNA-J, 20S proteasome alpha subunit B, translationally controlled tumor protein), ubiquitins (e.g., polyubiquitin, ubiquitin activating enzyme 2), among others. Downregulated genes were also identified, which likewise display identity with genes involved in several metabolic pathways. Differential expression of selected genes was validated by macroarray analysis and quantitative real-time polymerase chain reaction. The possible roles played by some of these genes in the viral infection cycle are discussed.

© 2009 The American Phytopathological Society