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The TIR Domain of TIR-NB-LRR Resistance Proteins Is a Signaling Domain Involved in Cell Death Induction

February 2009 , Volume 22 , Number  2
Pages  157 - 165

Michal R. Swiderski, Doris Birker, and Jonathan D. G. Jones

Sainsbury Laboratory, John Innes Centre, Norwich Research Park Colney Lane, Norwich, NR4 7UH, U.K.

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Accepted 2 October 2008.

In plants, the TIR (toll interleukin 1 receptor) domain is found almost exclusively in nucleotide-binding (NB) leucine-rich repeat resistance proteins and their truncated homologs, and has been proposed to play a signaling role during resistance responses mediated by TIR containing R proteins. Transient expression in Nicotiana benthamiana leaves of “TIR + 80”, the RPS4 truncation without the NB-ARC domain, leads to EDS1-, SGT1-, and HSP90-dependent cell death. Transgenic Arabidopsis plants expressing the RPS4 TIR+80 from either dexamethasone or estradiol-inducible promoters display inducer-dependent cell death. Cell death is also elicited by transient expression of similarly truncated constructs from two other R proteins, RPP1A and At4g19530, but is not elicited by similar constructs representing RPP2A and RPP2B proteins. Site-directed mutagenesis of the RPS4 TIR domain identified many loss-of-function mutations but also revealed several gain-of function substitutions. Lack of cell death induction by the E160A substitution suggests that amino acids outside of the TIR domain contribute to cell death signaling in addition to the TIR domain itself. This is consistent with previous observations that the TIR domain itself is insufficient to induce cell death upon transient expression.

Additional keywords:apoptosis.

© 2009 The American Phytopathological Society