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Comparative Analysis of Expression Profiles in Shoots and Roots of Tomato Systemically Infected by Tomato spotted wilt virus Reveals Organ-Specific Transcriptional Responses

December 2009 , Volume 22 , Number  12
Pages  1,504 - 1,513

Marco Catoni,1 Laura Miozzi,1 Valentina Fiorilli,2 Luisa Lanfranco,2 and Gian Paolo Accotto1

1Institute of Plant Virology, CNR, Strada delle Cacce, 73, 10135 Torino, Italy; 2Department of Plant Biology, University of Turin, Viale Mattioli, 10125 Torino, Italy

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Accepted 17 July 2009.

Tomato (Solanum lycopersicon), a model species for the family Solanaceae, is severely affected by Tomato spotted wilt virus (TSWV) worldwide. To elucidate the systemic transcriptional response of plants to TSWV infection, microarray experiments were performed on tomato. Parallel analysis of both shoots and roots revealed organ-specific responses, although the virus was present in similar concentration. In the shoots, genes related to defense and to signal transduction were induced, while there was general repression of genes related to primary and secondary metabolism as well as to amino acid metabolism. In roots, expression of genes involved in primary metabolism and signal transduction appear unaffected by TSWV infection, while those related to the response to biotic stimuli were induced and those associated to the response to abiotic stress were generally repressed or unaltered. Genes related to amino acid metabolism were unaffected, except for those involved in synthesis of secondary compounds, where induction was evident. Differential expression of genes involved in metabolism and response to ethylene and abscisic acid was observed in the two organs. Our results provide new insight into the biology of the economically important interaction between tomato and TSWV.

© 2009 The American Phytopathological Society