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Expression of Medicago truncatula Genes Responsive to Nitric Oxide in Pathogenic and Symbiotic Conditions

June 2008 , Volume 21 , Number  6
Pages  781 - 790

Alberto Ferrarini,1 Matteo De Stefano,1 Emmanuel Baudouin,2 Chiara Pucciariello,2 Annalisa Polverari,3 Alain Puppo,2 and Massimo Delledonne1

1Dipartimento Scientifico e Tecnologico, Università degli Studi di Verona, 37134 Verona, Italy; 2Interactions Plantes-Microorganismes et Santé Végétale, UMR INRA 1064/Université de Nice-Sophia Antipolis/CNRS 6192, 400, route des Chappes, BP 167, 06903 Sophia-Antipolis, France; 3Dipartimento di Scienze, Tecnologie e Mercati della Vite e del Vino, Università degli Studi di Verona, Villa Ottolini-Lebrecht, 37029 San Floriano di Valpolicella (VR), Italy

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Accepted 18 February 2008.

Nitric oxide (NO) is involved in diverse physiological processes in plants, including growth, development, response to pathogens, and interactions with beneficial microorganisms. In this work, a dedicated microarray representing the widest database available of NO-related transcripts in plants has been produced with 999 genes identified by a cDNA amplified fragment length polymorphism analysis as modulated in Medicago truncatula roots treated with two NO donors. The microarray then was used to monitor the expression of NO-responsive genes in M. truncatula during the incompatible interaction with the foliar pathogen Colletotrichum trifolii race 1 and during the symbiotic interaction with Sinorhizobium meliloti 1021. A wide modulation of NO-related genes has been detected during the hypersensitive reaction or during nodule formation and is discussed with special emphasis on the physiological relevance of these genes in the context of the two biotic interactions. This work clearly shows that NO-responsive genes behave differently depending on the plant organ and on the type of interaction, strengthening the need to consider regulatory networks, including different signaling molecules.

© 2008 The American Phytopathological Society