Link to home

A Single Amino Acid of NIaPro of Papaya ringspot virus Determines Host Specificity for Infection of Papaya

August 2008 , Volume 21 , Number  8
Pages  1,046 - 1,057

Kuan-Chun Chen,1 Chu-Hui Chiang,2 Joseph A. J. Raja,1 Fang-Lin Liu,1 Chun-Hsi Tai,1 and Shyi-Dong Yeh1

1Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C.; 2Department of Molecular Biotechnology, Da Yeh, University, Chang Hwa, Taiwan, R.O.C.

Go to article:
Accepted 2 April 2008.

Most strains of Papaya ringspot virus (PRSV) belong to type W, causing severe loss on cucurbits worldwide, or type P, devastating papaya in tropical areas. While the host range of PRSV W is limited to plants of the families Chenopodiaceae and Cucuribitaceae, PRSV P, in addition, infects plants of the family Caricaceae (papaya family). To investigate one or more viral genetic determinants for papaya infection, recombinant viruses were constructed between PRSV P-YK and PRSV W-CI. Host reactions to recombinant viruses indicated that the viral genomic region covering the C-terminal region (142 residues) of NIaVPg, full NIaPro, and N-terminal region (18 residues) of NIb, is critical for papaya infection. Sequence analysis of this region revealed residue variations at position 176 of NIaVPg and positions 27 and 205 of NIaPro between type P and W viruses. Host reactions to the constructed mutants indicated that the amino acid Lys27 of NIaPro determines the host-specificity of PRSV for papaya infection. Predicted three-dimensional structures of NIaPros of parental viruses suggested that Lys27 does not affect the protease activity of NIaPro. Recovery of the infected plants from certain papaya-infecting mutants implied involvement of other viral factors for enhancing virulence and adaptation of PRSV on papaya.

Additional keywords:host-specificity determinant.

© 2008 The American Phytopathological Society