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DsbB Is Required for the Pathogenesis Process of Xanthomonas campestris pv. campestris

August 2008 , Volume 21 , Number  8
Pages  1,036 - 1,045

Bo-Le Jiang,1,2 Jiao Liu,1 Li-Feng Chen,1 Ying-Ying Ge,1 Xiao-Hong Hang,1 Yong-Qiang He,1,2 Dong-Jie Tang,1,2 Guang-Tao Lu,1,2 and Ji-Liang Tang1,2

1College of Life Science and Technology, Guangxi University, China; 2Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization and The Key Laboratory of Microbial and Plant Genetic Engineering, 100 Daxue Road, Nanning, Guangxi 530004, China

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Accepted 22 April 2008.

The DsbA/DsbB oxidation pathway is one of the two pathways that catalyze disulfide bond formation of proteins in the periplasm of gram-negative bacteria. It has been demonstrated that DsbA is essential for multiple virulence factors of several animal bacterial pathogens. In this article, we present genetic evidence to show that the open reading frame XC_3314 encodes a DsbB protein that is involved in disulfide bond formation in periplasm of Xanthomonas campestris pv. campestris, the causative agent of crucifer black rot disease. The dsbB mutant of X. campestris pv. campestris exhibited attenuation in virulence, hypersensitive response, cell motility, and bacterial growth in planta. Furthermore, mutation in the dsbB gene resulted in ineffective type II and type III secretion systems as well as flagellar assembly. These findings reveal that DsbB is required for the pathogenesis process of X. campestris pv. campestris.

© 2008 The American Phytopathological Society