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Polyubiquitin Promoter-Based Binary Vectors for Overexpression and Gene Silencing in Lotus japonicus

April 2008 , Volume 21 , Number  4
Pages  375 - 382

Takaki Maekawa,1,2 Mitsumasa Kusakabe,2 Yoshikazu Shimoda,3 Shusei Sato,3 Satoshi Tabata,3 Yoshikatsu Murooka,2 and Makoto Hayashi1,2

1Institut für Genetik, Ludwig-Maximilians-Universität München, Maria-Ward-Str. 1a, 80638 München, Germany; 2Department of Biotechnology, Graduate school of Engineering, Osaka University, Yamadaoka 2-1, Suita Osaka 565-0871, Japan; 3Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan

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Accepted 4 December 2007.

In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using β-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus.

© 2008 The American Phytopathological Society