July
2007
, Volume
20
, Number
7
Pages
781
-
793
Authors
Trudy A.
Torto-Alalibo
,
1
Sucheta
Tripathy
,
1
Brian M.
Smith
,
1
Felipe D.
Arredondo
,
1
Lecong
Zhou
,
1
Hua
Li
,
1
Marcus C.
Chibucos
,
1
Dinah
Qutob
,
2
Mark
Gijzen
,
2
Chunhong
Mao
,
1
Bruno W. S.
Sobral
,
1
Mark E.
Waugh
,
3
Thomas K.
Mitchell
,
4
Ralph A.
Dean
,
4
and
Brett M.
Tyler
1
Affiliations
1Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, U.S.A.; 2Agriculture and Agri-Food Canada, 1391 Sandford Street, London, Ontario N5V 4T3, Canada; 3National Center for Genome Resources, Santa Fe, NM 87505, U.S.A.; 4Center for Integrated Fungal Research, North Carolina State University, Raleigh, NC 27695, U.S.A.
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RelatedArticle
Accepted 26 January 2007.
Abstract
Six unique expressed sequence tag (EST) libraries were generated from four developmental stages of Phytophthora sojae P6497. RNA was extracted from mycelia, swimming zoospores, germinating cysts, and soybean (Glycine max (L.) Merr.) cv. Harosoy tissues heavily infected with P. sojae. Three libraries were created from mycelia growing on defined medium, complex medium, and nutrient-limited medium. The 26,943 high-quality sequences obtained clustered into 7,863 unigenes composed of 2,845 contigs and 5,018 singletons. The total number of P. sojae unigenes matching sequences in the genome assembly was 7,412 (94%). Of these unigenes, 7,088 (90%) matched gene models predicted from the P. sojae sequence assembly, but only 2,047 (26%) matched P. ramorum gene models. Analysis of EST frequency from different growth conditions and morphological stages revealed genes that were specific to or highly represented in particular growth conditions and life stages. Additionally, our results indicate that, during infection, the pathogen derives most of its carbon and energy via glycolysis of sugars in the plant. Sequences identified with putative roles in pathogenesis included avirulence homologs possessing the RxLR motif, elicitins, and hydrolytic enzymes. This large collection of P. sojae ESTs will serve as a valuable public genomic resource.
JnArticleKeywords
Additional keyword:
effectors.
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ArticleCopyright
© 2007 The American Phytopathological Society