January
2007
, Volume
20
, Number
1
Pages
21
-
30
Authors
Ning
Zang
,
1
,
2
Dong-Jie
Tang
,
2
Mei-Liang
Wei
,
2
Yong-Qiang
He
,
2
Baoshan
Chen
,
2
Jia-Xun
Feng
,
2
Jing
Xu
,
2
Yong-Qi
Gan
,
2
Bo-Le
Jiang
,
2
and
Ji-Liang
Tang
2
Affiliations
1College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; 2Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning Guangxi 530004, China
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RelatedArticle
Accepted 2 July 2006.
Abstract
Macrophage infectivity potentiators (Mips) are FKBP domain-containing proteins reported as virulence factors in several human pathogens, such as members of genera Legionella, Salmonella and Chlamydia. The putative peptidyl-prolyl cis-trans isomerase (PPIase) encoded by XC2699 of the plant bacterial pathogen Xanthomonas campestris pv. campestris 8004 exhibits a 49% similarity at the aminoacid level to the Mip protein of Legionella pneumophila. This mip-like gene, XC2699, was overexpressed in Es-cherichia coli and the purified (His)6-tagged Mip-like protein encoded by XC2699 exhibited a PPIase activity specifically inhibited by FK-506. A mutation in the mip-like gene XC2699 led to significant reductions in virulence and replication capacity in the host plant Chinese radish (Raphanus sativus L. var. radiculus Pers.). Furthermore, the production of exopolysaccharide and the activity of extracellular proteases, virulence factors X. campestris pv. campestris, were significantly decreased in the mip-like mutant. These results reveal that the mip-like gene is involved in the pathogenesis of X. campestris pv. campestris through an effect on the production of these virulence factors.
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© 2007 The American Phytopathological Society