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Characterization of the Interaction Between the Bacterial Wilt Pathogen Ralstonia solanacearum and the Model Legume Plant Medicago truncatula

February 2007 , Volume 20 , Number  2
Pages  159 - 167

Fabienne Vailleau , 1 Elodie Sartorel , 1 Marie-Françoise Jardinaud , 1 Fabien Chardon , 2 Stéphane Genin , 2 Thierry Huguet , 1 Laurent Gentzbittel , 1 and Michel Petitprez 1

1INP-ENSAT, Laboratoire de Biotechnologie et Amélioration des Plantes, BP32607, 31326 Castanet-Tolosan cedex, France; 2Laboratoire des Interactions Plantes-Microorganismes, UMR CNRS/INRA 2594, BP52627, 31326 Castanet-Tolosan cedex, France

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Accepted 28 August 2006.

The soilborne pathogen Ralstonia solanacearum is the causal agent of bacterial wilt and attacks more than 200 plant species, including some legumes and the model legume plant Medicago truncatula. We have demonstrated that M. truncatula accessions Jemalong A17 and F83005.5 are susceptible to R. solanacearum and, by screening 28 R. solana-cearum strains on the two M. truncatula lines, differential interactions were identified. R. solanacearum GMI1000 infected Jemalong A17 line, and disease symptoms were dependent upon functional hrp genes. An in vitro root inoculation method was employed to demonstrate that R. solanacearum colonized M. truncatula via the xylem and intercellular spaces. R. solanacearum multiplication was restricted by a factor greater than 1 × 105 in the resistant line F83005.5 compared with susceptible Jemalong A17. Genetic analysis of recombinant inbred lines from a cross between Jemalong A17 and F83005.5 revealed the presence of major quantitative trait loci for bacterial wilt resistance located on chromosome 5. The results indicate that the root pathosystem for M. truncatula will provide useful traits for molecular analyses of disease and resistance in this model plant species.

Additional keywords: QTL.

© 2007 The American Phytopathological Society