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Identification of 118 Arabidopsis Transcription Factor and 30 Ubiquitin-Ligase Genes Responding to Chitin, a Plant-Defense Elicitor

August 2007 , Volume 20 , Number  8
Pages  900 - 911

Marc Libault , 1 Jinrong Wan , 1 Tomasz Czechowski , 2 Michael Udvardi , 2 and Gary Stacey 1

1National Center for Soybean Biotechnology, Divisions of Plant Science and Biochemistry, Department of Molecular Microbiology and Immunology, Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, U.S.A.; 2Max-Planck Institute of Molecular Plant Physiology, Potsdam, Germany

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Accepted 7 March 2007.

Chitin, found in the cell walls of true fungi and the exoskeleton of insects and nematodes, is a well-established elicitor of plant defense responses. In this study, we analyzed the expression patterns of Arabidopsis thaliana transcription factor (TF) and ubiquitin-ligase genes in response to purified chitooctaose at different treatment times (15, 30, 60, 90, and 120 min after treatment), using both quantitative polymerase chain reaction and the Affymetrix Arabidopsis whole-genome array. A total of 118 TF genes and 30 ubiquitin-ligase genes were responsive to the chitin treatment. Among these genes, members from the following four TF families were overrepresented: APETALA2/ethylene-reponsive element binding proteins (27), C2H2 zinc finger proteins (14), MYB domain-containing proteins (11), and WRKY domain transcription factors (14). Transcript variants from a few of these genes were found to respond differentially to chitin, suggesting transcript-specific regulation of these TF genes.

Additional keyword: PAMP

© 2007 The American Phytopathological Society