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The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fusca

April 2007 , Volume 20 , Number  4
Pages  430 - 440

Diego Romero , 1 Antonio de Vicente , 1 Rivo H. Rakotoaly , 2 Samuel E. Dufour , 2 Jan-Willem Veening , 3 Eva Arrebola , 1 Francisco M. Cazorla , 1 Oscar P. Kuipers , 3 Michel Paquot , 2 and Alejandro Pérez-García 1

1Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos s/n, E-29071 Málaga, Spain; 2Unité de Chemie Biologie Industrielle, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passaje des Déportés 2, B-5030 Gembloux, Belgium; 3Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands

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Accepted 24 November 2006.

Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the mode of action involved in their biocontrol performance. Cell-free supernatants showed antifungal activities very close to those previously reported for vegetative cells. Identification of three lipopeptide antibiotics, surfactin, fengycin, and iturin A or bacillomycin, in butanolic extracts from cell-free culture filtrates of these B. subtilis strains pointed out that antibiosis could be a major factor involved in their biocontrol ability. The strong inhibitory effect of purified lipopeptide fractions corresponding to bacillomycin, fengycin, and iturin A on P. fusca conidia germination, as well as the in situ detection of these lipopeptides in bacterial-treated melon leaves, provided interesting evidence of their putative involvement in the antagonistic activity. Those results were definitively supported by site-directed mutagenesis analysis, targeted to suppress the biosynthesis of the different lipopeptides. Taken together, our data have allowed us to conclude that the iturin and fengycin families of lipopep-tides have a major role in the antagonism of B. subtilis toward P. fusca.

Additional keywords: antifungals, biological control, cucurbits.

© 2007 The American Phytopathological Society