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Genetics of Symbiosis in Lotus japonicus: Recombinant Inbred Lines, Comparative Genetic Maps, and Map Position of 35 Symbiotic Loci

January 2006 , Volume 19 , Number  1
Pages  80 - 91

Niels Sandal , 1 Thomas Rørby Petersen , 1 Jeremy Murray , 2 Yosuke Umehara , 3 Bogumil Karas , 2 Koji Yano , 4 Hirotaka Kumagai , 3 Makoto Yoshikawa , 4 Katsuharu Saito , 5 Masaki Hayashi , 6 Yasuhiro Murakami , 3 Xinwang Wang , 3 Tsuneo Hakoyama , 7 Haruko Imaizumi-Anraku , 3 Shusei Sato , 8 Tomohiko Kato , 8 Wenli Chen , 3 Md. Shakhawat Hossain , 3 Satoshi Shibata , 3 Trevor L. Wang , 9 Keisuke Yokota , 1 Knud Larsen , 1 Norihito Kanamori , 1 Esben Madsen , 1 Simona Radutoiu , 1 Lene H. Madsen , 1 Talida Gratiela Radu , 1 Lene Krusell , 1 , 10 Yasuhiro Ooki , 11 Mari Banba , 11 Marco Betti , 12 Nicolas Rispail , 13 Leif Skøt , 13 Elaine Tuck , 13 Jillian Perry , 14 Satoko Yoshida , 14 , 15 Kate Vickers , 14 Jodie Pike , 14 Lonneke Mulder , 14 Myriam Charpentier , 14 Judith Müller , 14 Ryo Ohtomo , 16 Tomoko Kojima , 16 Shotaro Ando , 16 Antonio J. Marquez , 12 Peter M. Gresshoff , 17 Kyuya Harada , 6 Judith Webb , 13 Shingo Hata , 11 Norio Suganuma , 7 Hiroshi Kouchi , 3 Shinji Kawasaki , 3 Satoshi Tabata , 8 Makoto Hayashi , 4 Martin Parniske , 14 , 15 Krzysztof Szczyglowski , 2 Masayoshi Kawaguchi , 5 and Jens Stougaard , 1

1Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark; 2Agriculture and Agri-Food Canada, SCPFRC, 1391 Sandford Street, London, Ontario NV5 4T3, Canada; 3National Institute of Agrobiological Sciences, 2-1-2 Kannon-dai, Tsukuba, Ibaraki, 305-8602, Japan; 4Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; 5Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033; 6Faculty of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan; 7Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan; 8Kazusa DNA Research Institute, 1532-3 Yana, Kisarazu, Chiba 292, Japan; 9John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, England; 10Molecular Plant Nutrition Group, Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Golm, Germany; 11Laboratory of Plant Biochemistry, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; 12Facultad de Química, Universidad de Sevilla, Apartado 553, 41080 Sevilla, Spain; 13IGER, Department of Plant Genetics and Breeding, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB, Wales, U.K.; 14Sainsbury Laboratory, Norwich Research Park, Colney, Norwich, NR4 7UH, England; 15Genetics Institute, Ludwig Maximilians Universität (LMU), Maria-Ward-Str. 1a, D-80638, Germany; 16National Institute of Livestock and Grassland Science, 768 Senbonmatsu, Nasushiobara, Tochigi 329-2793, Japan; 17ARC Centre of Excellence for Integrative Legume Research, University of Queensland, Brisbane Qld 4072, Australia


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Accepted 12 September 2005.

Development of molecular tools for the analysis of the plant genetic contribution to rhizobial and mycorrhizal symbiosis has provided major advances in our understanding of plant-microbe interactions, and several key symbiotic genes have been identified and characterized. In order to increase the efficiency of genetic analysis in the model legume Lotus japonicus, we present here a selection of improved genetic tools. The two genetic linkage maps previously developed from an interspecific cross between L. japonicus Gifu and L. filicaulis, and an intraspecific cross between the two ecotypes L. japonicus Gifu and L. japonicus MG-20, were aligned through a set of anchor markers. Regions of linkage groups, where genetic resolution is obtained preferentially using one or the other parental combination, are highlighted. Additional genetic resolution and stabilized mapping populations were obtained in recombinant inbred lines derived by a single seed descent from the two populations. For faster mapping of new loci, a selection of reliable markers spread over the chromosome arms provides a common framework for more efficient identification of new alleles and new symbiotic loci among uncharacterized mutant lines. Combining resources from the Lotus community, map positions of a large collection of symbiotic loci are provided together with alleles and closely linked molecular markers. Altogether, this establishes a common genetic resource for Lotus spp. A web-based version will enable this resource to be curated and updated regularly.


Additional keywords: symbiotic mutants.

The American Phytopathological Society, 2006