January
2006
, Volume
19
, Number
1
Pages
80
-
91
Authors
Niels
Sandal
,
1
Thomas Rørby
Petersen
,
1
Jeremy
Murray
,
2
Yosuke
Umehara
,
3
Bogumil
Karas
,
2
Koji
Yano
,
4
Hirotaka
Kumagai
,
3
Makoto
Yoshikawa
,
4
Katsuharu
Saito
,
5
Masaki
Hayashi
,
6
Yasuhiro
Murakami
,
3
Xinwang
Wang
,
3
Tsuneo
Hakoyama
,
7
Haruko
Imaizumi-Anraku
,
3
Shusei
Sato
,
8
Tomohiko
Kato
,
8
Wenli
Chen
,
3
Md. Shakhawat
Hossain
,
3
Satoshi
Shibata
,
3
Trevor L.
Wang
,
9
Keisuke
Yokota
,
1
Knud
Larsen
,
1
Norihito
Kanamori
,
1
Esben
Madsen
,
1
Simona
Radutoiu
,
1
Lene H.
Madsen
,
1
Talida Gratiela
Radu
,
1
Lene
Krusell
,
1
,
10
Yasuhiro
Ooki
,
11
Mari
Banba
,
11
Marco
Betti
,
12
Nicolas
Rispail
,
13
Leif
Skøt
,
13
Elaine
Tuck
,
13
Jillian
Perry
,
14
Satoko
Yoshida
,
14
,
15
Kate
Vickers
,
14
Jodie
Pike
,
14
Lonneke
Mulder
,
14
Myriam
Charpentier
,
14
Judith
Müller
,
14
Ryo
Ohtomo
,
16
Tomoko
Kojima
,
16
Shotaro
Ando
,
16
Antonio J.
Marquez
,
12
Peter M.
Gresshoff
,
17
Kyuya
Harada
,
6
Judith
Webb
,
13
Shingo
Hata
,
11
Norio
Suganuma
,
7
Hiroshi
Kouchi
,
3
Shinji
Kawasaki
,
3
Satoshi
Tabata
,
8
Makoto
Hayashi
,
4
Martin
Parniske
,
14
,
15
Krzysztof
Szczyglowski
,
2
Masayoshi
Kawaguchi
,
5
and
Jens
Stougaard
,
1
Affiliations
1Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark; 2Agriculture and Agri-Food Canada, SCPFRC, 1391 Sandford Street, London, Ontario NV5 4T3, Canada; 3National Institute of Agrobiological Sciences, 2-1-2 Kannon-dai, Tsukuba, Ibaraki, 305-8602, Japan; 4Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; 5Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033; 6Faculty of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan; 7Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan; 8Kazusa DNA Research Institute, 1532-3 Yana, Kisarazu, Chiba 292, Japan; 9John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, England; 10Molecular Plant Nutrition Group, Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Golm, Germany; 11Laboratory of Plant Biochemistry, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; 12Facultad de Química, Universidad de Sevilla, Apartado 553, 41080 Sevilla, Spain;
13IGER, Department of Plant Genetics and Breeding, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB, Wales, U.K.; 14Sainsbury Laboratory, Norwich Research Park, Colney, Norwich, NR4 7UH, England; 15Genetics Institute, Ludwig Maximilians Universität (LMU), Maria-Ward-Str. 1a, D-80638, Germany; 16National Institute of Livestock and Grassland Science, 768 Senbonmatsu, Nasushiobara, Tochigi 329-2793, Japan; 17ARC Centre of Excellence for Integrative Legume Research, University of Queensland, Brisbane Qld 4072, Australia
Go to article:
RelatedArticle
Accepted 12 September 2005.
Abstract
Development of molecular tools for the analysis of the plant genetic contribution to rhizobial and mycorrhizal symbiosis has provided major advances in our understanding of plant-microbe interactions, and several key symbiotic genes have been identified and characterized. In order to increase the efficiency of genetic analysis in the model legume Lotus japonicus, we present here a selection of improved genetic tools. The two genetic linkage maps previously developed from an interspecific cross between L. japonicus Gifu and L. filicaulis, and an intraspecific cross between the two ecotypes L. japonicus Gifu and L. japonicus MG-20, were aligned through a set of anchor markers. Regions of linkage groups, where genetic resolution is obtained preferentially using one or the other parental combination, are highlighted. Additional genetic resolution and stabilized mapping populations were obtained in recombinant inbred lines derived by a single seed descent from the two populations. For faster mapping of new loci, a selection of reliable markers spread over the chromosome arms provides a common framework for more efficient identification of new alleles and new symbiotic loci among uncharacterized mutant lines. Combining resources from the Lotus community, map positions of a large collection of symbiotic loci are provided together with alleles and closely linked molecular markers. Altogether, this establishes a common genetic resource for Lotus spp. A web-based version will enable this resource to be curated and updated regularly.
JnArticleKeywords
Additional keywords:
symbiotic mutants.
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ArticleCopyright
The American Phytopathological Society, 2006