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Redox-Active Pyocyanin Secreted by Pseudomonas aeruginosa 7NSK2 Triggers Systemic Resistance to Magnaporthe grisea but Enhances Rhizoctonia solani Susceptibility in Rice

December 2006 , Volume 19 , Number  12
Pages  1,406 - 1,419

David De Vleesschauwer , 1 Pierre Cornelis , 2 and Monica Höfte 1

1Laboratory of Phytopathology, Faculty of Bioscience Engineering, Ghent University, Coupure links, 653, B-9000 Gent, Belgium; 2Department of Molecular and Cellular Interactions, Flanders Interuniversity Institute of Biotechnology, Laboratory of Microbial Interactions, Vrije Universiteit Brussel, Building E6.6, Pleinlaan 2, 1050 Brussels, Belgium

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Accepted 10 July 2006.

Pseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the salicylic acid-derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed to consistently reduce sheath blight (Rhizoctonia solani). Only mutations interfering with pyocyanin production led to a significant decrease in induced systemic resistance (ISR) to M. grisea, and in trans complementation for pyocyanin production restored the ability to elicit ISR. Intriguingly, pyocyanin-deficient mutants, unlike the wild type, triggered ISR against R. solani. Hence, bacterial pyocyanin plays a differential role in 7NSK2-mediated ISR in rice. Application of purified pyocyanin to hydroponically grown rice seedlings increased H2O2 levels locally on the root surface as well as a biphasic H2O2 generation pattern in distal leaves. Co-application of pyocyanin and the antioxidant sodium ascorbate alleviated the opposite effects of pyocyanin on rice blast and sheath blight development, suggesting that the differential effectiveness of pyocyanin with respect to 7NSK2-triggered ISR is mediated by transiently elevated H2O2 levels in planta. The cumulative results suggest that reactive oxygen species act as a double-edged sword in the interaction of rice with the hemibiotroph M. grisea and the necrotroph R. solani.

Additional keywords: hypersensitive response , oxidative burst , Pyricularia grisea , rhizobacteria .

© 2006 The American Phytopathological Society