December
2006
, Volume
19
, Number
12
Pages
1,385
-
1,393
Authors
Georgina
Estrada-Navarrete
,
1
Xochitl
Alvarado-Affantranger
,
1
Juan-Elías
Olivares
,
1
Claudia
Díaz-Camino
,
1
Olivia
Santana
,
1
Enrique
Murillo
,
1
Gabriel
Guillén
,
1
Nayeli
Sánchez-Guevara
,
1
Jorge
Acosta
,
2
Carmen
Quinto
,
1
Dongxue
Li
,
3
Peter M.
Gresshoff
,
3
and
Federico
Sánchez
1
Affiliations
1Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62271, México; 2Campo Experimental del Valle de México, Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias, Texcoco, Edo. de México, México; 3ARC Centre of Excellence for Integrative Legume Research, The University of Queensland, St. Lucia, Brisbane QLD 4072, Australia
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RelatedArticle
Accepted 7 July 2006.
Abstract
A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, landraces, and cultivars and for three other species belonging to the genus Phaseolus: P. coccineus, P. lunatus, and P. acutifolius. Induced hairy roots are robust and grow quickly. The transformation frequency is between 75 and 90% based on the 35-S promoter-driven green fluorescent protein and β-glu-curonidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal determinate nodules that fix nitrogen as efficiently as inoculated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiology, root metabolism, and root--microbe interactions.
JnArticleKeywords
Additional keywords:
common bean
,
T-DNA
,
transgenic nodules
.
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ArticleCopyright
© 2006 The American Phytopathological Society