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Agrobacterium rhizogenes Transformation of the Phaseolus spp.: A Tool for Functional Genomics

December 2006 , Volume 19 , Number  12
Pages  1,385 - 1,393

Georgina Estrada-Navarrete , 1 Xochitl Alvarado-Affantranger , 1 Juan-Elías Olivares , 1 Claudia Díaz-Camino , 1 Olivia Santana , 1 Enrique Murillo , 1 Gabriel Guillén , 1 Nayeli Sánchez-Guevara , 1 Jorge Acosta , 2 Carmen Quinto , 1 Dongxue Li , 3 Peter M. Gresshoff , 3 and Federico Sánchez 1

1Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62271, México; 2Campo Experimental del Valle de México, Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias, Texcoco, Edo. de México, México; 3ARC Centre of Excellence for Integrative Legume Research, The University of Queensland, St. Lucia, Brisbane QLD 4072, Australia

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Accepted 7 July 2006.

A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, landraces, and cultivars and for three other species belonging to the genus Phaseolus: P. coccineus, P. lunatus, and P. acutifolius. Induced hairy roots are robust and grow quickly. The transformation frequency is between 75 and 90% based on the 35-S promoter-driven green fluorescent protein and β-glu-curonidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal determinate nodules that fix nitrogen as efficiently as inoculated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiology, root metabolism, and root--microbe interactions.

Additional keywords: common bean , T-DNA , transgenic nodules .

© 2006 The American Phytopathological Society