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An Iron Uptake Operon Required for Proper Nodule Development in the Bradyrhizobium japonicum-Soybean Symbiosis

September 2005 , Volume 18 , Number  9
Pages  950 - 959

Heather P. Benson , Eric Boncompagni , and Mary Lou Guerinot

Department of Biological Sciences; 6044 Gilman, Dartmouth College, Hanover, NH 03755 U.S.A.

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Accepted 22 April 2005.

Rhizobia live in the soil or enter into a nitrogen-fixing symbiosis with a suitable host plant. Each environment presents different challenges with respect to iron acquisition. The soybean symbiont Bradyrhizobium japonicum 61A152 can utilize a variety of siderophores (Fe[III]-specific ligands). Purification of iron-regulated outer membrane proteins had previously allowed the cloning of a gene, fegA, from B. ja-ponicum 61A152, whose predicted protein shares significant amino acid similarity with known TonB-dependent siderophore receptors. Here, we show that fegA is in an operon with a gene, fegB, that is predicted to encode an inner membrane protein. Characterization of fegAB and fegB mutants shows that both fegA and fegB are required for utilization of the siderophore ferrichrome. Whereas the fegB mutant forms a normal symbiosis, the fegAB mutant has a dramatic phenotype in planta. Six weeks after inoculation with a fegAB strain, soybean nodules do not contain leghemoglobin and do not fix nitrogen. Infected cells contain few symbiosomes and are filled with vesicles. As ferrichrome is a fungal siderophore not likely to be available in nodules, the symbiotic defect suggests that the fegAB operon is serving a different function in planta, possibly one involved in signaling between the two partners.

Additional keywords: nitrogen fixation.

© 2005 The American Phytopathological Society