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Expression Profiling Soybean Response to Pseudomonas syringae Reveals New Defense-Related Genes and Rapid HR-Specific Downregulation of Photosynthesis

November 2005 , Volume 18 , Number  11
Pages  1,161 - 1,174

Jijun Zou , 1 Sandra Rodriguez-Zas , 2 Mihai Aldea , 3 Min Li , 1 Jin Zhu , 1 Delkin O. Gonzalez , 1 Lila O. Vodkin , 1 Evan DeLucia , 3 Steven J. Clough 1 , 4

1Department of Crop Sciences, University of Illinois; 2Department of Animal Sciences, University of Illinois; 3Department of Plant Biology, University of Illinois, Urbana, IL 61801, U.S.A.; 4USDA-ARS, Urbana, IL 61801, U.S.A.

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Accepted 14 July 2005.

Transcript profiling during susceptible (S) and hypersensitive response-associated resistance (R) interactions was determined in soybean (Glycine max). Pseudomonas syringae pv. glycinea carrying or lacking the avirulence gene avrB, was infiltrated into cultivar Williams 82. Leaf RNA was sampled at 2, 8, and 24 h postinoculation (hpi). Significant changes in transcript abundance were observed for 3,897 genes during the experiment at P ≤ 0.000005. Many of the genes showed a similar direction of increase or decrease in abundance in both the S and R responses, but the R response generally showed a significantly greater degree of differential expression. More than 25% of these responsive genes had not been previously reported as being associated with pathogen interactions, as 704 had no functional annotation and 378 had no homolog in National Center for Biotechnology Information databases. The highest number of transcriptional changes was noted at 8 hpi, including the downregulation of 94 chloroplast-associated genes specific to the R response. Photosynthetic measurements were consistent with an R-specific reduction in photosystem II operating efficiency (ΦPSII) that was apparent at 8 hpi for the R response with little effect in the S or control treatments. Imaging analyses suggest that the decreased ΦPSII was a result of physical damage to PSII reaction centers.

Additional keywords: compatible, genomic, incompatible, oxidative burst.

© 2005 The American Phytopathological Society